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Consistency is important for better results

V., Santos S

Redox western Trichloroacetic acidprecipitated cell extracts (Delaunay et al , 2002) were dissolved in SB buffer (TrisCl (100 mM, pH 8.8), EDTA (10 mM) and SDS (1%)) containing 30 mM 4acetamido4maleimidylstilbene2,2disulphonic acid (AMS, Invitrogen), or 50 mM Nethylmaleimide and incubated at 37C for 2 h

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10.1016/j.ejmech.2021.113401 82 JornayvazF

The tumor volume and body weight were evaluated for 20 days