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Description
Nature 1978 276: 5683 276:7576

Ice crystal formation physically disrupts the molecular structure of both the water and benzyl alcohol, rendering the preservative ineffective and compromising sterility

The membranes were blocked with 5 % skim milk (dilution in TBS with 0.05 % Tween 20 (TBST)) and incubated with primary anti-bodies (1:1000, dilution in TBST) for overnight at 4C

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