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Notably, RT-qPCR analysis showed a sharp increase in OCT4 mRNA expression, a marker of pluripotent stem cells and essential for TIC self-renewal [33], in the colonies formed in 3D gels relative to 2D culture conditions (Fig

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For instance, a study published in the journal Hepatology indicated that ibuprofen administration in mice led to decreased hepatic glutathione levels, suggesting that NSAIDs could contribute to glutathione depletion under certain conditions

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