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In cervical cancer (HeLa) cells, apigenin induce apoptosis via two distinct pathways: one involves down-regulating Bcl-2 protein expression, while the other activates p53 to varying degrees, leading to the induction of p21, fatty acid synthase (Fas) expression and ultimately apoptosis [80]

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Consequently, 45 l of the aqueous phase was transferred to a new sample tube prior to reduction with 2.5 l of 50 mM TCEP in AA for 30 min at 37 C and re-alkylation with 2.5 l of 100 mM d5-NEM

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