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Osteophytes and fracture calluses share developmental milestones and are diminished by unloading

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The result is typically slower weight loss but the same total weight loss over a longer period, with potentially less metabolic shock and less hair follicle disruption

doi: 10.1186/s12879-018-2991-2

For whole cell and tissue samples, samples were first lysed in ice-cold radioimmunoprecipitation assay (RIPA) buffer supplemented with a cocktail of protease

J Dermatol Sci 47:141149 Hou YC, Guo ZM, Li J, Wang PG (1996) Seleno compounds and glutathione peroxidase catalyzed decomposition of S-Nitrosothiols