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Image Credits: all eating ice cream by salem elizabeth is licensed under CC BY 2.0 Figure 5.21

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Adenine nucleotides (Sigma-Aldrich) were added to the pipette solution shortly before the experiments and the pH was additionally adjusted

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This is the standard beyond-use date for most compounded injectables

Starvation assays L4 animals were washed off plates and rinsed three times with M9