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Estimation of proinflammatory cytokines and cortisol levels Serum cortisol, interleukin 1 beta (IL-1), tumor necrosis factor-alpha (TNF-), and interleukin 6 (IL-6) levels were estimated using commercial ELISA kits provided from Cusabio (Wuhan, China) (CSB-E08487f, CSB-E13259Fh, CSB-E13254Fh, CSB-E13258Fh for cortisol, IL-1, TNF-, IL-6, respectively) based on the manufacturer instructions using a microplate reader (Bio-Tek, VT, USA) at absorbance of 450 nm and expressed as ng/mL for cortisol and pg/mL for IL-1, TNF-, and IL-6 (Leng et al

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