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The rinsed cell pellets were re-suspended in Milli-Q water and incubated at 95 C for 3 min, then cooled immediately on ice for 3 min and the supernatant was separated by centrifugation (16,000 g , 1 min)

Int J Mol Sci 24(20):15344 Dogaru CB, Muscurel C, Du C, Stoian I (2023b) Alphabet selenoproteins: their characteristics and physiological roles

Melatonin ameliorates endocrine dysfunction and defective sperm integrity associated with highfat dietinduced obesity in male Wistar rats

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O

An additional clinically significant group of enzymes that requires tetrahydrobiopterin as cofactor are the nitric oxide synthase (NOS) family of enzymes

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