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Description
A linear gradient of 0.1% TFA in water (Solvent A) and 0.1% TFA in acetonitrile (ACN) (Solvent B) was used, starting at 99% A with 1% B and increasing per minute at a flow rate of 1 mL/min and detection at 215 nm
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Tip: Drinking enough fluids throughout the day is essential
A., & Morales-Gonzlez, J
Time lines are different
Exposure is over 90 min in medium containing sufficient histidine to support two cell divisions
