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The study administered a dosage of 500mg twice per day for six weeks in a randomized, placebo-controlled, and crossover-designed trial

Glutamine, which is a nonessential amino acid (NEAA) due to the endogenous glutamine biosynthesis pathway, is currently considered essential in cancer cells because transformed cells consume glutamine at a rate exceeding that of glutamine biosynthesis 5

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As a dietary supplement take 1-2 sachets per day

Subsequently, cells were Fixed with 4% paraformaldehyde for 15 min at room temperature, permeabilized with 0.1% Triton X-100 for 5 min, stained with EdU solution (Thermo Fisher Scientific) for 30 min