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Briefly, tissue sections were incubated with primary antibodies for 1 h at room temperature (RT), followed by incubation with the secondary HRP-coupled antibody (10 min, RT)

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B12 Missing from Vegan and Vegetarian Diets Scientists surmise that plant-eating humans once got adequate amounts of cobalamin from small amounts of dirt found on the surface of plants, in much the same way that plant-eating animals like cattle acquire the nutrient

However, they observed a higher risk of developing bladder 109, 110, renal, and endocrine cancers 111, 112

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