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Mechanistically, intranasally administered agents may be absorbed through the olfactory epithelium or trigeminal nerve endings and transported to the brain via extracellular diffusion and intracellular axonal pathways, which may partially bypass BBB-related constraints (Raghav et al., 2023

Conversely, genetic inhibition of autophagy (e.g., Atg7 knockdown) reduced apoptosis and preserved spermatogenesis, suggesting that excessive autophagy can be harmful to germ cells 42

AO/PI staining and Annexin-V FITC assays confirmed apoptotic cell death, with cell cycle arrest at the sub-G1 phase

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Chronic stress can deplete glutamine stores, making the gut more vulnerable to damage
and (3) the recruitment of mixed-lineage kinase-like protein (MLKL), which, upon phosphorylation by the RIPK1-RIPK3 complex, assembles into a new complex, called the necrosome (Sun et al., 2012
