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The semi-quantitative PCR was set for the genes (NLRP3, caspase 1, ASC, gasdermin D, IL-1, Nf-Bp65, IL-6, GAPDH), which contained 100 l reaction volume consisting of 1x PCR buffer, a variable concentration of MgCl 2 , cDNA, forward and reverse primers, 200 M of dNTPs, and volume makeup with water