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doi:10.3760/cma.j.issn.1009-2587.2019.08.014

The reaction was initiated by the addition of 190 l reaction buffer, adjusted to pH 7.4 by titration with K 2 HPO 4 (40 mM) and KH 2 PO 4 (10 mM) stock solutions, supplemented with NADH (100 M) and sodium pyruvate (600 M)

These nanocarriers typically incorporate redox-sensitive chemical bonds, such as disulfide linkages, which remain stable in blood circulation but undergo cleavage in the reductive tumour microenvironment, leading to structural transformation and payload discharge

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Consistently, by ChIP assay, we detected significant NRF2 binding in Nqo1 and Slc7a11 loci, along with induced association of H3K4Me4 and H3K9Ac after DMF treatment (Fig