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Lenti-X 293T cells were transfected with pKLV2-mCherry_TTR_GFPGFPv3 (described above) together with psPAX2 (Addgene, #11260) and pMD2G (Addgene, #11259) to produce lentivirus
When we discuss the gut microbiome, we're essentially talking about the gastrointestinal tract (GI tract), in particular the intestines
