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Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific), and then further analyzed by Protein Thermal Shift Software v1.3 (Thermo Fisher Scientific)

Some indirect data support the possibility that LTPs also play a role in ME initiation in barley (Vrinten et al., 1999 and our study)

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