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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

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One of the possible mechanisms thought to be related to brain overgrowth is the increased blood concentrations of brain-derived neurotrophic factor (BDNF) in newborns who were later diagnosed with ASD (Hazlett et al., 2011

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