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Multiple experiments indicate that GCL is the rate-limiting enzyme in the plant glutathione biosynthesis (Foyer et al., 1995

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Under physiological conditions Trx is fully reduced and interacts with pro-apoptotic proteins, such as apoptosis signaling kinase 1 (ASK1), the tumor suppressor phosphatase and tensin homologue deleted on chromosome 10 (PTEN), activator protein 1 (AP-1) and p53 [8789]