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Moreover, metformin exerts protective effects on skeletal tissue by preventing glucose- and lipotoxicity-induced osteoblast apoptosis and mitigating type 2 diabetes mellitus-associated bone deterioration

Aylward Juliet L

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Then 80% of the supernatant was transferred to a new tube and 2 l of concentrated ammonia solution added, followed by 100 l of 4 mg/ml ethyl bromoacetate (Oakwood chemicals) in ACN and samples vortex mixed
ETC function was examined on frozen muscle and cultured fibroblasts (Baylor Medical Genetics Laboratory)

Biology, 11 (8), 1140
