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BOTOX Cosmetic is approved for moderate to severe glabellar lines, lateral canthal lines, forehead lines, and platysma bands in adults

Processing parameters must be optimized to maintain bioactivity while ensuring safety and shelf life, with techniques such as microencapsulation and freeze-drying widely applied (281)

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3.3 Determination of bromelain activity Casein and azocasein are the most used substrates for the spectrophotometric determination of bromelain activity

However, there are only a few reports of GGT enzyme immobilization that might be attributed to the heterodimeric composition of the enzyme that makes it difficult to remain in the active conformation after immobilization

Meanwhile, the vector backbone of pTH19Kr plasmid was produced by PCR with primers (5-caagcttggcgtaatcatgg and 5-taatgctctgccagtgttac)