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Redox potential measurements Cyclic voltammetry measurements were carried out with a BioLogicSP200 potentiostat with EC-Lab software

Resin-embedded tissues were sectioned, mounted, and stained with hematoxylin and eosin (H&E) prior to imaging

Structure-activity Relationship Study of RSL3-based GPX4 Degraders and Its Potential Noncovalent Optimization

Western blot analysis of GPX4 expression in HC neutrophils (n = 3) when cultured with 20% HC serum or SLE serum supplemented with Cl-amidine (Cl, 100 M), APX-115 (APX) (pan-NADPH oxidase (NOX) inhibitor, 20 M), and GSK2795039 (GSK) (NOX2 inhibitor, 10 M)

GSH was measured by its reaction with the chromogenic substrate DTNB, resulting in a yellow TNB product with an absorption maximum of 412 nm

10.1186/s13018-019-1242-6 86 PetekM.SikiricP.AnicT.BuljatG.SeparovicJ.Stancic-RokotovD.et al (1999)