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For histological examination, one side of the ovarian and uterine tissues was fixed in 4% paraformaldehyde, while the other side was quickly frozen in liquid nitrogen and stored at 80 C for later RNA extraction

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5.2 GABA and GABA/GAD65-alum alters Th-1 cytokine response in children with recent onset T1D In the same cohort as the GABA/GAD-alum study (82), the potential immune effects of GABA treatment, with or without GAD65 immunization, were examined (89)

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The animals were housed in a controlled environment (23 1 C, 12-h light/dark cycle) and had free access to food and water

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