glutathione reduction assay for quantitative determination of and glutathione disulfide levels using enzymatic recycling method Glutathione Peroxidase (GSH-Px) Activity Assay

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Shiitake mushrooms that were sun-dried indoors at 26C for 36 h exhibited reduced levels of total phenolic content (93.4 to 3.9 mg/100 g in fresh shiitake), total antioxidants (109.5 to 5.4 mg/100 g in fresh shiitake), and scavenging activity (43.2 to 6.6%) in comparison to microwave and freeze-drying techniques (98)

Rosmarin et al., 2024)

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Neuropathy or neurological disease: Although evidence is mixed, injections are often given when deficiency is linked to nerve pain or cognitive symptoms