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However, it's important to check with the manufacturer for any additives or stabilizers used in the injection solution

After centrifugation at 1000 x g for 10 min, the supernatant (S1) was centrifuged for 20 min at 17.500 x g to yield the supernatant S2 and the pellet P2 (crude synaptosomal fraction)

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Additionally, the S/T-3A mutation 37 , which disables serine/threonine phosphorylation sites at residues 356, 361 and 375 of XKR8, but not the 2DA mutants, enabled XKR8 KO HL-60 cells to expose PS in response to apoptosis induced by doxorubicin and NET induction by PMA, thereby restored the NET formation (Fig