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Natural compounds from medicinal plants usually have low water solubility and high lipophilicity, which results in inadequate absorption and bioavailability

After washing three times with PBS, the liver tissues were homogenized by adding 0.1 M Tris-HCl buffer (pH 7.4) in a volume 10 times the tissue weight

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The cells were then incubated with different formulations containing 250 nM of Cy5-labeled AsiG in a binding buffer (200 L) for 1 h on ice

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