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After stimulation, cells were washed once with 1x PBS and then collected in 150 l 2x Laemmli Sample Buffer supplemented with 5% 2-Mercaptoethanol
Statements Author contributions X-hL: Data curation, Formal analysis, Funding acquisition, Methodology, Writing original draft
After cultivation for 24 h, cell viability was investigated with CellTiter 96 Aqueous One Solution (Promega Corporation)
doi: 10.1016/0003-2697(87)90135-7
[DOI] [PubMed] [Google Scholar] 122.Tshionyi M., Shay E., Lunde E., Lin A., Han K.Y., Jain S., Chang J.H., Azar D.T
Egg, chicken, grains, beef and fish are common culprits
