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The direct way is mainly the direct action of high energy X-rays on the DNA double strand of the cell, causing it to break and inducing cell cycle arrest, senescence and cell death (Santivasi and Xia, 2014

In this regard, isolated hepatocytes of the healthy and tumor model mice were treated with 500 l of 10 mM of H 2 DCFDA for 45 min at 37 C

11.4 In vitro tumor models using dECM-based on tumor-on-a-chip Tumor-on-a-chip models are devices that integrate microfluidics, tissue engineering, and microfabrication to replicate key features of tumor physiology

doi: 10.1016/j.cmet.2011.11.013 32 CaputaGCastoldiAPearceEJ

1c,d and 3f and Extended Data Figs

Additional factors include excessive alcohol consumption, smoking, stress, gastric hyperacidity, diet, genetics, and radiotherapy (Sostres, Gargallo, and Lanas 2013 )