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Sample Preparation Animals were fasted overnight and for clinical chemistry blood samples from each rat were collected from the eyes by retro-orbital puncture using blood capillary tubes without heparin as per requirement under mild ether anesthesia blood samples were incubated at room temperature for 10 minutes and left to clot then centrifuged at 3000 r.p.m for 10 min and the serum were collected, serum was separated and kept in clean stopper plastic vial at 80C until the analysis of serum parameters

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In experimental systems, this compound is used to study melanocortin receptor signaling and peptide-receptor interactions involved in pigmentation pathways

Two copies of FMO3 mutations are generally needed to cause trimethylaminuria