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H.WalduraC.HerrmannW.GraeberS.et al (2017)

Another kinase that is linked to AMPK is Cyclic AMP-dependent Protein Kinase A (PKA), which is upregulated in response to starvation and plays a pro-longevity role though its involvement in lipolysis (Schmeisser et al., 2019)

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For NanoLuc assay measurements, the homogenate (10 L) was diluted in reporter lysis buffer (40 L) and sample (50 L) was further diluted in NanoLuc substrate (50 L) [Nano-Glo luciferase assay, 5000x dilution from stock, Promega Cat#N1110] and the luminescence were measured through an open filter