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2017;9(8) doi: 10.3390/nu9080795

RT-qPCR assays, RNA-seq, and bioinformatic analysis Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturers instructions

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It suggests that a higher amount of Pax6 is needed within the cortical primordium to repress hem fate than to repress Gsx2, suggesting that some Pax6 targets are also differentially sensitive to its dosage