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Electrophoretic mobility shift assay The recombinant proteins GSTECT12 were separately diluted to a series of concentrations ranging from 0 to 2 M in binding buffer (10 mM HEPES-KOH, pH 8.0, 50 mM KCl, 1 mM EDTA, 0.05% (v/v) Triton X-100, 5% (v/v) glycerol, 10 g ml 1 salmon DNA, 1 mM DTT and 40 U ml 1 Ribolock RNase inhibitor (Thermo Fisher Scientific))

F.BouljihadM.KimH.TrostL

To further verify genotypes of HCV, phylograms were constructed with the reference stains for the core and NS5B regions (Fig

The distal plate is then carefully freed from the underlying bed and elevated from it distally, with the proximal plate and proximal fold acting as a hinge

Measurements were taken on days 10, 15, and 20 following ST injection

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