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To simplify purification, affinity purification tags are often fused to a recombinant protein of interest.The most popular tag is the polyhistidine (6xHis) tag.Some proteins, like eukaryotic proteins may not exhibit proper protein activity or folding if expressed in E.coli

Design Type(s) parallel group design transcription profiling by array design protein expression profiling Measurement Type(s) transcription profiling assay protein expression profiling metabolite profiling Technology Type(s) microarray platform ELISA nuclear magnetic resonance assay Factor Type(s) disease stage obesity Sample Characteristic(s) Homo sapiens serum liver Machine-accessible metadata file describing the reported data (ISA-Tab format)