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Sixty-eight male C57BL/6 mice were fed either a standard or AGE-rich diet, then treated for 35 days and mated

GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Protein refolding Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating

Mechanistic investigations have implicated oxidative stress, inflammatory responses, and apoptosis as critical components in the etiology of CP-induced POF, although the exact process by which this ovarian toxicity occurs remains unclear
