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4 Insulin resistance often involves an increase in lipid storage in muscle and/or liver tissue which leads to an impairment in insulin signaling and energy homeostasis, as well as increases in plasma levels of glucose and free fatty acids

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Briefly, the paraffin sections were rehydrated and incubated in 0.06% potassium permanganate (KMnO 4 ) solution for 15 min, washed in distilled water, and transferred to a Fluoro-Jade solution for 30 min and washed and observed with a fluorescence microscope