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Plasma concentration was plotted using WinNonlin

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While clinical studies have demonstrated its fat-reducing potential, AOD-9604 has not been fully validated as an FDA-approved weight loss therapy

Briefly, cells pretreated with 4 mM glutamine (Gln) and 0 mM Gln, respectively, were cross-linked with formaldehyde at 37 C for 10 min, followed by the addition of glycine solution to quench the cross-linking reaction