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doi: 10.1016/j.jenvman.2014.02.024

2.2 Refolding and Purification Strategy for SRCRD Expression in the Supernatant The cell pellets were resuspended with lysis buffer (20 mM TrisHCl (pH 8.0), 200 mM NaCl, and 10 mM imidazole) and sonicated to break the cells using a Digital Sonifier (Branson)

doi: 10.1038/s41467-018-04072-4 49 LinkC

(25767408, 21970774) Toxicity from acetylcysteine is therefore clearly possible

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