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Absorbance at 450 nm was then measured using a microplate reader
Its effects accumulate quietly over weeks

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Devos D, Labreuche J, Rascol O, Corvol J-C, Duhamel A, Guyon Delannoy P, et al

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The rats were housed in plastic cages (56 animals per cage) under controlled conditions of temperature (25 2 C) and light cycle (12 h light, 12 h dark), with standard food pellets and tap water provided ad libitum
