glutathione stransferase deficiency Genetic of S-Transferase P Increases Myocardial Sensitivity to Ischemia–Reperfusion Injury Glutathione S-transferase and MRP1 form

Med J Babylon 2022;19:99

Altered protein function includes changes in enzyme catalytic activity, cytoskeletal organisation and cell signal transduction

For the amplification of GLUT-4 and Ppia , specific primers were used with the following real-time PCR steps: an initial denaturation step followed by 40 cycles of denaturation, annealing, and extension

The cells were then washed once in ice-cold PBS, trypsinized, pelleted, and then homogenized in mitochondrial isolation buffer (0.21 M mannitol, 0.07 M sucrose, 0.1 mM EDTA, 1 mM EGTA, 10 mM Tris HCl, 0.5% BSA, pH 7.4 using KOH)

If for some reason this fails we can then try one of the other tests

iPSCs, induced Pluripotent Stem Cells