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10.1016/j.bbi.2020.07.005 40 MassariC

Heliyon 5:e01710

The study results indicate that the crude methanolic extract and 12-ursene 2-diketone are capable of carrying out a natural anti-inflammatory activity at sites where chronic inflammation is present by switching off the pro-inflammatory cytokines and mediators, which initiate the process

The injection technique is straightforward but must be performed correctly to ensure full dose delivery and minimize discomfort

Quantitative reverse-transcription PCR (qRT-PCR) Total RNA extraction, cDNA synthesis, quality and quantity analysis, and qRT-PCR were performed as described previously (Zhang et al

Statistical analyses All statistical analyses were performed using GraphPad Prism version 8 (GraphPad Software www.graphpad.com)