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PCR amplicons were separated by electrophoresis on 1% agarose gel (40 min, 100 V), stained with gelred (Biotium, So Paulo, SP, Brazil), and visualized under ultraviolet light

However, as in all intensive animal exploitation, fish culture presents different alterations and pathologies affecting fish welfare, causing great economic losses to the aquaculture sector

Iron accumulation-induced ROS, such as superoxide anion (O 2 -) and hydroxyl radical (OH), possess an unpaired electron at their outer orbit which allows them to react with all cellular components including proteins, lipids and nucleic acid

The broad substrate utilization spectrum of K

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At these sites, 5-GABA A Rs are predominantly involved in generating a tonic inhibitory current [63,64,65] to regulate the excitability of pyramidal neurons [66, 67] and decrease network excitability