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After 24 h, the HFF-1 cells in the well plates were imaged using phase contrast microscopy (Zeiss, Axio, Germany) for growth appearance, adherence and confluence
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Research findings point to the capacity of busulfan to induce cellular senescence in various cell types, including mesenchymal stem cells, fibroblasts, and the cell lines of human osteosarcoma cells U2OS and MG63 [65,66,67]
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