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SM had been used as one of the bioactive ingredients in the clinical fracture treatment of traditional Chinese medicine for many years

Click chemistry and biotin affinity precipitation Cells were lysed in IP Lysis buffer (20 mM HEPES-KOH, 150 mM NaCl, and 0.5% v/v Triton-100x) containing protease inhibitor cocktail, and treated with 10 mM sodium borohydride (Sigma-Aldrich, 452882) for 30 min

Symptoms include upper stomach pain, fever, jaundice, and pale-colored stools

Protein extraction and Western blotting analysis Protein was extracted at different developmental stages and from different organs of adult female ticks during blood-feeding

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scatter (cells dissociation due to metalloprotease production), which often leads to metastasis