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Gene expression assay via Gpcr in fibroblasts and keratinocytes treated with Astaxanthin and Glutathione

Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)

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[DOI] [PMC free article] [PubMed] [Google Scholar] Tew K.D., Townsend D.M