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By quantifying how much of your protein was in your elution, and analyzing how much, if any, of your protein was in the flow-through, you can figure out if you used an appropriate amount of glutathione resin

There are many glutathione molecules conjugated to each agarose bead, so multiple GST-fusion proteins can bind to the same bead (Figure 3)

There have been a few slight modifications, however the number of categories and substances that feature remain the same as the 2015 List, as our table shows

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