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For Western blotting analysis, cell lysates were cleared by centrifugation at 10,000 g at 4 C

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[DOI] [PubMed] [Google Scholar] 28.Stanley TL, Chen CY, Branch KL, Makimura H, Grinspoon SK: Effects of a growth hormone-releasing hormone analog on endogenous GH pulsatility and insulin sensitivity in healthy men