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Briefly, sterile 0.3% oxgall and 100 g/mL cholesterol PEG 600 were added to freshly prepared de-Man-Rogosa-Sharpe (MRS) broth, inoculated with respective LAB isolates (1% v/v), maintained at 37 C for 24 h and viability was also determined by the spread plate method

Meanwhile, other studies have examined changes in the metallome over aging in a variety of tissues (Zhang et al., 2020

Statistical analyses Data were collected from 3 independent experiments and expressed as mean standard deviation (SD)

How this might affect TCR tuning or agonist selected cells, such as invariant natural killer T cells (iNKT cells), remains unknown
