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The extracted RNA was then treated with DNase (Ambion Life Technologies #AM1907) for an hour at 37C and used for downstream processing

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All treatments were performed in pre-warmed (37C) differentiation medium with reduced NGF- content (5 ng/ml) or with buffered Hank's solution (Capricorn Scientific GmbH, 5.26 mM KCl, 0.43 mM KH 2 PO 4 , 134.2 mM NaCl, 4.09 mM NaHCO 3 , 0.33 mM Na 2 HPO 4 , 5.44 mM glucose, 2 mM CaCl 2 , and 20 mM HEPES, pH 7.4

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