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Transcribed image text: Problem 2: N-acetyl para-benzoquinone (NAPQI) is an oxidized metabolite of acetaminophen formed in the liver

The process must be performed under aseptic conditions using sterile bacteriostatic water for injection (not saline, not PBS, not deionized water)

(6)Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (6) Preparation of Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (5)Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Asp(otBu)-OH1.1gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In the Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin in step (5), add decapping reagent (20% piperidine in DMF solution), react at room temperature for 5 minutes, drain, and add decapping reagent at normal temperature React for 20 minutes, wash with isopropanol for 3 times, DMF for 3 times, DMF for redistilled for 2 times, drain, add Fmoc-Asp(otBu)-OH1.1g, DIEA0.4ml, TBTU0.56g, HOBT0 .2g, DMF10ml normal temperature reaction for 1.5 hours, drained with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with DMF evaporated, and dried to obtain Fmoc-Asp(otBu)-Asp(otBu )-Ala-Gly-Leu-Val-WangResin

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In this state, glucose cannot be converted into pyruvate, reducing the amount of acetyl-CoA available for the TCA cycle and leading to depletion of ATP and activation of death pathways