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doi: 10.1038/onc.2017.216

The synergistic effect of nutrients in whole foods often enhances bioavailability compared to isolated supplement forms

However, the metabolites produced by microorganisms are thought to affect the physiological health of the host 29

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Each PCR reaction was carried out with Taq DNA Polymerase (Invitrogen, Carlsbad, California, United States) and consisted of the following reaction mixture: 0.3 M primers, 1/500th of the cDNA reaction (corresponds to cDNA derived from 10 ng of total RNA), 125 M dNTPs, 1.5 mM MgCl 2 , and 1X reaction buffer (20 mM Tris pH 8.4, 50 mM KCl)