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Proper aseptic technique includes: Cleaning the vial septum with appropriate antiseptic (typically 70% isopropyl alcohol) before each access Using a sterile needle and syringe for each withdrawal Maintaining sterile conditions throughout the preparation process Avoiding touch contamination of the vial septum or needle Following institutional or professional protocols for multi-dose vial management Consequence of Improper Technique: Failure to maintain aseptic technique can result in microbial contamination of the vial contents, potentially leading to serious infection if contaminated solution is administered

It is also very important for red blood cell formation, nervous system and nerve cell function, and DNA synthesis

Hooshmand B et al., Neurology 2010 B12 status and brain volume in elderly

Association of vitamin B(12) deficiency in people with type 2 diabetes on metformin and without metformin: A multicenter study, Karachi, Pakistan

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It contains 0.9 percent benzyl alcohol as a preservative